Information

7.3: Mitosis - Biology


This image provides a graphic illustration of the problem. It shows a bit (no more than 3%) of the single molecule of DNA released from a single human chromosome. (The chromosome was treated to remove its histones). Remembering that this is 3% of the DNA of only one of the 46 chromosomes in the human diploid cell, you can appreciate the problem faced by the cell of how to separate without error these great lengths of DNA without creating horrible tangles.

The solution to this problem is:

  1. Duplicate each chromosome during the S phase of the cell cycle.
  2. This produces dyads, each made up of 2 identical sister chromatids. These are held together by a ring of proteins called cohesin.
  3. Condense the chromosomes into a compact form. This requires ATP and protein complexes called condensins.
  4. Separate the sister chromatids and
  5. Distribute these equally between the two daughter cells.

The Right Material for the Job

Building a house is a big job and one that requires a lot of different materials for specific purposes. As you can see in Figure 7.4.1, many different types of materials are used to build a complete house, but each type of material fulfills certain functions. You wouldn’t use insulation to cover your roof, and you wouldn’t use lumber to wire your home. Just as a builder chooses the appropriate materials to build each aspect of a home (wires for electrical, lumber for framing, shingles for roofing), your body uses the right cells for each type of role. When many cells work together to perform a specific function, this is termed a tissue .


AP Lab 3 Sample 4 Mitosis

All cells come from other cells. New cells are formed during cell division which involves both the replication of the cell’s nucleus and division of the cytoplasm. The two kinds of cellular division are mitosis and meiosis. Mitosis usually makes body cells, somatic cells. Mitosis is used in adult cells for asexual reproduction, regeneration, and the maintenance and repair of body parts. The process called meiosis makes gametes, sperm and eggs, and spores in plants. Gamete or spore cells have half the chromosomes that the parent cell has.

Mitosis is the first of the cell divisions studied in this lab. It is easily observed in cells that are growing at a fast paces such as whitefish blastula or onion root tips, which are used in this lab. The onion root tips have the highest percentage of cells going through mitosis. The whitefish blastula is formed directly after the egg is fertilized. This is a period of a fast paced growth and numerous cellular divisions where mitosis can be observed. Just before mitosis the cell is in interphase, a part of the cell cycle where the cell has a distinct nucleus and nucleoli. Next is prophase, where the chromatin thickens into distinct chromosomes and the nuclear envelope breaks open releasing them into the cytoplasm. The first signs of the spindle apparatus begin to appear. Next the cell begins metaphase, where the spindle attaches to the centromere of each chromosome pair and moves them to the middle of the cell. This level position is called the metaphase plate. Then anaphase begins when the chromatids are separated and pulled to the opposite poles. The final stage is telophase where the nuclear envelope is reformed and the chromosomes gradually uncoil. Cytokinesis then may occur forming a cleavage furrow and then the two daughter cells will separate.

Meiosis is more complex and involves two nuclear divisions. The two divisions are called Meiosis I and Meiosis II. These two divisions result in the production of four haploid gametes. This process allows increased genetic variation due to crossing over where genes can be exchanged. The process, like mitosis, depends on interphase to replicate the DNA. Meiosis begins with prophase I. In this stage, homologous chromosomes move together to form a tetrad. This is where crossing over occurs resulting in the recombination of genes. Metaphase I moves the tetrads to the metaphase plate in the middle of the cell, and anaphase I reduces the tetrads to their original two stranded form and moves them to opposite poles. Telophase I then prepares the cell for its second division. Meiosis II is just like mitosis except that the daughter cells are haploid instead of diploid. DNA replication does not occur in interphase II, and prophase II, metaphase II, anaphase II, and telophase II occur as usual. The only change is the number of chromosomes.

Mitosis is easily observed in the whitefish blastula and the onion root tip. Meiosis and crossing over occurs in the production of gametes, in animals, and spores, in plants.

The materials used in this lab are as follows: light microscopes, prepared slides of whitefish blastula and onion root tips, pencil, and paper.

The materials used in this lab are as follows: light microscopes, prepared slides of onion root tips, paper, and pencil.

The materials used in this section of the lab are as follows: a chromosome simulation kit, pencil and paper.

The materials used in this section of the lab are as follows: light microscopes, prepared slides of Sordaria fimicola, pencil, and paper.

Observe prepared slides of whitefish blastula and onion root tips under the 10X and 40X objectives. Sketch and identify each section of cell division.

Observe every cell and determine what stage the cell is in. Count at least 200 cells total, separating them into groups of the same phase. Consider it takes 24 hours for the onion root-tip cells to complete the cell cycle.

Use the lab book to show how to make the chromosomes. The simulation kit has plenty of beads to use. There are red and yellow beads to be used to show the different chromatids. There is also a piece that resembles half of a centromere which has a magnet to connect to another one.

Use a light microscope to observe the prepared slide and record all data.

The sketches below show the phases of mitosis for the onion root-tip.


Watch the video: Biology - Secret of Life - - Analysing a Gene 2 - DNA Sequencing (January 2022).